Differential effect of nitric oxide on thrombospondin-1-, PDGF- and fibronectin-induced migration of vascular smooth muscle cells

作者：Seymour Keri; Han Xuan; Sadowitz Benjamin; Maier Kristopher G; Gahtan Vivian^*

来源：American Journal of Surgery, 2010, 200(5): 615-619.

DOI：10.1016/j.amjsurg.2010.07.018

• Nitric oxide
• Thrombospondin-1
• Migration
• Vascular smooth muscle cells
• Extracellular matrix
• PDGF

BACKGROUND Neointimal hyperplasia involves the migration of medial vascular smooth mu,cle cells (VSMCs) in response to arterial injury Thrombospondine-1 (TSP1) platelet derived growth fa( tor (PDGF) and fibronectin (Fn) Induce VSMC migration Nitric oxide (NO) limits VSMC migration The hypothesis of this study is that NO would dose dependently inhibit TSP1 Induced PDGF-induced and Fn induced VSMC chemotaxis
METHODS VSMCs were pretreated with serum free media or the NO donors diethylenetriamine NONOate or S-nitroso-N-acetyl D,L-penicillamine Chemotaxis to TSP1 PDGF or Fn was determined Analysis of variance with post hoc testing was done P values < 05 were considered significant
RESULTS PDGF TSPI and Fn induced VSMC chemotaxis NO donors inhibited chemotaxic of VSMCs to PDGF in a concentration dependent manner NO donors had a variable effect on TSP1 induced chemotaxis NO donors did not inhibit Fn induced chemotaxis
CONCLUSION The complex interactions of these proteins in vivo will need to be considered when developing NO dependent therapies for neointimal hyperplasia Published by Elsevier Inc

• 出版日期2010-11