Applications of NMR to structure determination of RNAs large and small

作者：Barnwal Ravi P; Yang Fan; Varani Gabriele^*

来源：Archives of Biochemistry and Biophysics, 2017, 628: 42-56.

DOI：10.1016/j.abb.2017.06.003

• RNA
• Isotope labeling
• Structure
• NMR spectroscopy

Nuclear magnetic resonance (NMR) spectroscopy is a powerful tool to investigate the structure and dynamics of RNA, because many biologically important RNAs have conformationally flexible structures, which makes them difficult to crystallize. Functional, independently folded RNA domains, range in size between simple stem-loops of as few as 10-20 nucleotides, to 50-70 nucleotides, the size of tRNA and many small ribozymes, to a few hundred nucleotides, the size of more complex RNA enzymes and of the functional domains of non-coding transcripts. In this review, we discuss new methods for sample preparation, assignment strategies and structure determination for independently folded RNA domains of up to 100 kDa in molecular weight.

• 出版日期2017-8-15