APOLIPOPROTEIN E4 ALLELE IS ASSOCIATED WITH SUBSTANTIAL CHANGES IN THE PLASMA LIPIDS AND HYALURONIC ACID CONTENT IN PATIENTS WITH NONALCOHOLIC FATTY LIVER DISEASE

作者：Stachowska E^*; Maciejewska D; Ossowski P; Drozd A; Ryterska K; Banaszczak M; Milkiewicz M; Raszeja Wyszomirska J; Slebioda M; Milkiewicz P; Jelen H

来源：Journal of Physiology and Pharmacology, 2013, 64(6): 711-717.

• non-alcoholic fatty liver disease
• non-alcoholic steatohepatitis
• apolipoprotein E
• fatty acids
• 5-oxo-6,8,11,14-eicosatetraenoic acid
• liquid chromatography/tandem mass spectrometry
• gas chromatography

Fat may affect progression of liver damage in patients with non-alcoholic fatty liver disease (NAFLD). In this study we characterize the state of lipid metabolism in 22 patients with NAFLD and different Apo-E variants. Total concentration of plasma total fatty acids was quantified by gas chromatography, while their derivatives by liquid chromatography/tandem mass spectrometry (LC ESI MS/MS). The ratio of plasma saturated fatty acid to monounsaturated fatty acid increased, whereas the ratio of polyunsaturated fatty acids to saturated fatty acids was reduced in Apo-E4 carriers. Simultaneously, the levels of individual plasma linoleic, arachidonic, and alpha linolenic acids significantly increased in subjects with the Apo-E4 allele. The 15-lipoxygenase metabolite, 13-hydroxyoctadecadienoic acid, was significantly higher in Apo-E3 carriers (p%26lt;0.006). 5-oxo-6,8,11,14-eicosatetraenoic acid was significantly elevated in Apo-E4 carriers (p%26lt;0.009). A significant difference in hyaluronic acid concentration (p%26lt;0.0016) as well as predicted advanced fibrosis (using the BARD scoring system) was found in Apo-E4 carriers (p%26lt;0.01). We suggest that a distinct mechanism of fibrosis between Apo E alleles. In Apo-E4 carriers, an elevation in 5-oxo-6,8,11,14-eicosatetraenoic acid synthesis and fatty acid dysfunction may induce fibrosis, while an inflammatory process may be the main cause of fibrosis in Apo-E3 carriers.

• 出版日期2013-12