Toll-Like Receptor Responses in IRAK-4-Deficient Neutrophils

作者：van Bruggen Robin^*; Drewniak Agata; Tool Anton T J; Jansen Machiel; van Houdt Michel; Geissler Judy; van den Berg Timo K; Chapel Helen; Kuijpers Taco W

来源：Journal of Innate Immunity, 2010, 2(3): 280-287.

DOI：10.1159/000268288

• Neutrophil
• IRAK-4
• Toll-like receptors

Human neutrophils were found to express all known Toll-like receptors (TLRs) except TLR3 and TLR7. IRAK-4-deficient neutrophils were tested for their responsiveness to various TLR ligands. Essentially all TLR responses in neutrophils, including the induction of reactive oxygen species generation, adhesion, chemotaxis and IL-8 secretion, were found to be dependent on IRAK-4. Surprisingly, the reactivity towards certain established TLR ligands, imiquimod and ODN-CpG, was unaffected by IRAK-4 deficiency, demonstrating their activity is independent of TLR. TLR-4-dependent signaling in neutrophils was totally dependent on IRAK-4 without any major TRIF-mediated contribution. We did not observe any defects in killing capacity of IRAK-4-deficient neutrophils for Staphylococcus aureus, Eschenchia cob and Candida albicans, suggesting that microbial killing is primarily TLR independent.

• 出版日期2010