Development of multiple-locus variable-number tandem-repeat analysis for the molecular subtyping of Enterobacter sakazakii

作者：Mullane N R; Ryan M; Iversen C; Murphy M; O'Gaora P; Quinn T; Whyte P; Wall P G; Fanning S^*

来源：Applied and Environmental Microbiology, 2008, 74(4): 1223-1231.

DOI：10.1128/AEM.01726-07

The genomic content of Enterobacter sakazakii strain ATCC BAA-894 was analyzed for variable-number tandem repeats (VNTRs). In this study we report the development of a multiple-locus VNTR analysis (MLVA) strategy for the subtyping of E. sakazakii. The method is based on a GeneScan analysis of four VNTR loci labeled with multiple fluorescent dyes. This approach was applied to a collection of 112 isolates representing all 16 of the currently defined E. sakazakii biogroups. MLVA successfully discriminated among these isolates and compared favorably with pulsed-field gel electrophoresis. The method was relatively fast and easy to perform. The potential value of MLVA as an epidemiological tool is discussed.

• 出版日期2008-2