In Vitro Fertilization and Embryo Culture Strongly Impact the Placental Transcriptome in the Mouse Model

作者：Fauque Patricia^*; Mondon Francoise; Letourneur Franck; Ripoche Marie Anne; Journot Laurent; Barbaux Sandrine; Dandolo Luisa; Patrat Catherine; Wolf Jean Philippe; Jouannet Pierre; Jammes Helene; Vaiman Daniel

来源：PLos One, 2010, 5(2): e9218.

DOI：10.1371/journal.pone.0009218

Background: Assisted Reproductive Technologies (ART) are increasingly used in humans; however, their impact is now questioned. At blastocyst stage, the trophectoderm is directly in contact with an artificial medium environment, which can impact placental development. This study was designed to carry out an in-depth analysis of the placental transcriptome after ART in mice.
Methodology/Principal Findings: Blastocysts were transferred either (1) after in vivo fertilization and development (control group) or (2) after in vitro fertilization and embryo culture. Placentas were then analyzed at E10.5. Six percent of transcripts were altered at the two-fold threshold in placentas of manipulated embryos, 2/3 of transcripts being down-regulated. Strikingly, the X-chromosome harbors 11% of altered genes, 2/3 being induced. Imprinted genes were modified similarly to the X. Promoter composition analysis indicates that FOXA transcription factors may be involved in the transcriptional deregulations.
Conclusions: For the first time, our study shows that in vitro fertilization associated with embryo culture strongly modify the placental expression profile, long after embryo manipulations, meaning that the stress of artificial environment is memorized after implantation. Expression of X and imprinted genes is also greatly modulated probably to adapt to adverse conditions. Our results highlight the importance of studying human placentas from ART.

• 出版日期2010-2-15