Novel bioconversion products of andrographolide by Aspergillus ochraceus and their cytotoxic activities against human tumor cell lines

作者：He, Xiangjiu^*; Wang, Yihai; Hu, Hui; Wu, Yixuan; Zeng, Xiaobin

来源：Journal of Molecular Catalysis B: Enzymatic , 2011, 68(1): 89-93.

DOI：10.1016/j.molcatb.2010.09.017

• Andrographolide
• Aspergillus ochraceus
• Hydroxylation
• Microbial conversion
• Cytotoxicity

Andrographolide (1) a major labdane diterpenoidal constituent of a famous traditional Chinese of Andrographis paniculata exhibits a wide spectrum of biological activities including antibacterial, anti-inflammatory and antitumor properties Bioconversion of andrographolide (1) by Aspergillus ochraceus (ATCC 1008) was investigated Five bioconversion products were isolated and identified Their structures were identified to be 8 beta-hydroxy-8(17)-dihydroandrographolide (2), 8 beta-hydroxy-8(17)-dihydro-14-deoxy-11 12-didehydroandrographohde (3) 80-hydroxy-8(17)-dihydro-14-deoxy-11 12-didehydroandrographolide 19-oic acid (4). 14-deoxy-11 12-didehydroandrographolide (5), and 14-deoxy-11 12-didehydroandrographolide 19-oic acid (6) Metabolites 2-4 were novel compounds The proposed biosynthetic pathways of andrographolide by A ochraceus were drawn Most

• 出版日期2011-1
• 单位武汉大学