Fabrication and Evaluation of Polycaprolactone-Poly(hydroxybutyrate) or Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Dual-Leached Porous Scaffolds for Bone Tissue Engineering Applications

作者：Thadavirul Napaphat; Pavasant Prasit; Supaphol Pitt^*

来源：Macromolecular Materials and Engineering, 2017, 302(3): UNSP 1600289.

DOI：10.1002/mame.201600289

• PCL scaffold
• PHB
• PHBV
• polymer leaching method
• solvent casting/particulate leaching method

Polycaprolactone (PCL) blend with poly(hydroxybutyrate) (PHB) or poly(3-hydroxybutyrate-co-3- hydroxyvalerate) (PHBV) dual-leached scaffolds are prepared by using the solvent casting and salt-polymer- leaching technique. The blending of the PHB and PHBV in PCL scaffolds results in decreased porosities of the scaffolds, and the water absorption capacities of the scaffolds also decrease. The compressive modulus of the PCL-PHB and PCL-PHBV dual-leached scaffolds is greatly increased by the blending of PHB or PHBV matrix. An indirect cytotoxicity evaluation of all scaffolds with mouse fibroblastic cells (L929) and mouse calvaria-derived pre-osteoblastic cell (MC3T3-E1) indicates that all dual-leached scaffolds are posed as nontoxic to cells. Both PCL-PHB and PCL-PHBV dual-leached scaffolds are supported by the attachment of MC3T3-E1 at significantly higher levels to tissue culture polystyrene plate (TCPS) and are able to support the proliferation of MC3T3-E1 at higher levels to that cells on TCPS and PCL scaffolds. For mineralization, cells cultured on surfaces of PCL-PHB and PCL-PHBV dual-leached scaffolds show higher mineral deposition than on TCPS and PCL scaffold.

• 出版日期2017-3