Development of a Model of Pediatric Lung Failure Pathophysiology

作者：Trahanas John M; Alghanem Fares; Ceballos Muriel Catalina; Hofman Hayley R; Xu Alice; Deatrick Kristopher B; Cornell Marie S; Rojas Pena Alvaro^*; Bartlett Robert H; Hirschl Ronald B

来源：ASAIO Journal, 2017, 63(2): 216-222.

DOI：10.1097/MAT.0000000000000463

• lung failure
• animal model
• pulmonary hypertension

A pediatric artificial lung (PAL) is under development as a bridge to transplantation or lung remodeling for children with end-stage lung failure (ESLF). To evaluate the efficiency of a PAL, a disease model mimicking the physiologic derangements of pediatric ESLF is needed. Our previous right pulmonary artery (rPA) ligation model (rPA-LM) achieved that goal, but caused immediate mortality in nearly half of the animals. In this study, we evaluated a new technique of gradual postoperative right pulmonary artery occlusion using a Rummel tourniquet (rPA-RT) in seven (25-40 kg) sheep. This technique created a stable model of ESLF pathophysiology, characterized by high alveolar dead space (58.0% +/- 3.8%), pulmonary hypertension (38.4 +/- 2.2 mm Hg), tachypnea (79 +/- 20 breaths per minute), and intermittent supplemental oxygen requirement. This improvement to our technique provides the necessary physiologic derangements for testing a PAL, whereas avoiding the problem of high immediate perioperative mortality.

• 出版日期2017-4