Phylogenetic diversity of cultivable butyrate-producing bacteria from pig gut content and feces

作者：Li X^*; Hojberg O; Canibe N; Jensen B B

来源：Journal of Animal Science, 2016, 94(suppl_3): 377-381.

DOI：10.2527/jas2015-9868

• butyrate
• cultivable bacteria
• diversity
• pigs

Butyrate is a preferred energy source for colonocytes and is considered crucial for maintaining colonic health in humans and animals. To investigate the diversity of cultivable butyrate-producing bacteria in pig gut, bacteria were isolated from intestinal digesta (Exp. 1) and feces (Exp. 2) of finishers. Based on 16S rRNA gene sequencing, 2,762 isolates were categorized into 122 operational taxonomic units (OTUs). Representative isolates of 31 OTUs produced butyrate. Complete 16S rRNA gene sequences of the 31 OTUs were subjected to phylogenetic analysis, and based on a level of < 97% 16S rRNA gene sequence similarity with their nearest validly named neighbors, 15 of the OTUs appeared to represent novel species. The abundance of the cultivable butyrate-producing community was 8.3%, 10.7%, 17.2%, and 7.0% in the ileum, cecum, colon, and feces, respectively. Butyrate producers within clostridial clusters IX (Acidaminococcus and Megasphaera) and XIVa (Coprococcus/Eubacterium/Roseburia) were found to be the most abundant. Members of the Bacteroidetes and Fusobacteria phyla were also identified as butyrate producers. Notably, high-fiber diet was correlated with lower abundance of Acidaminococcus fermentans, Clostridium perfringens, and Clostridium rectum but higher abundance of the commensal Megasphaera elsdenii.

• 出版日期2016-9