Differential Gene Expression Identified by Suppression Subtractive Hybridization During Late Ripening of Fruit in Oil Palm (Elaeis guineensis Jacq.)

作者：Al Shanfari Ahmed B; Abdullah Siti Nor Akmar^*; Saud Halimi Mohd; Omidvar Vahid; Napis Suhami

来源：Plant Molecular Biology Reporter, 2012, 30(3): 768-779.

DOI：10.1007/s11105-011-0383-5

• Elaeis guineensis Jacq.
• cDNA
• Fruit ripening
• Mesocarp
• Suppression subtractive hybridization

A forward suppression subtractive hybridization (SSH) library was constructed to identify genes involved in fruit ripening in an oil palm (Elaeis guineensis Jacq). The suppression was performed with cDNA extracted from fruits at 12 weeks after anthesis (w.a.a.) as %26quot;driver%26quot; and at 17 w.a.a. as %26quot;tester%26quot;. A single-pass sequencing from the 5%26apos;-end of 2,112 randomly selected cDNA clones resulted in 2,019 high-quality expressed sequence tags (ESTs) with an average reading length of 383 bp. Clustering of the 2,019 EST sequences using StackPACK showed 20 unigenes consisting of 9 contigs and 11 singletons. Among the edited EST sequences, 1,109 (14 unigenes) had significant matches with protein sequences in the public databases. The matched ESTs were further classified into six putative cellular functions including unclassified proteins (38.0%), cell rescue and defense (33.8%), proteins with binding functions (27.7%), biogenesis of cellular component (0.3%), metabolism (0.1%), and cell cycle (0.1%). The expression levels of the 20 obtained unigenes were analyzed using RT-PCR. At 17 w.a.a., 20 unigenes were significantly up-regulated. The findings in this study will contribute to a better understanding of the molecular mechanisms involved in fruit ripening in oil palm.

• 出版日期2012-6