科研之友
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摘要
BACKGROUNDMicrobial enhanced oil recovery is a potential technology to enhance crude oil recovery from depleted oil reservoirs and solve stagnant petroleum production. Relevant studies have mainly focused on the use of bacteria, with less attention paid to the potential of fungi. In this study, the efficiency of fungal extracellular enzymes for crude oil degradation and their feasibility for use in enhanced oil recovery were evaluated. @@@ RESULTSSix fungal cultures of Aspergillus spp. were isolated from oil-contaminated surface soil samples from an oilfield. The performance of fungal extracellular enzymes to degrade crude oil was investigated using solid enzyme preparations. All the enzyme preparations efficiently degraded the alkane, aromatic, resins, asphaltene, and decreased the viscosity of crude oil to varying degrees (max 59.0%). Enzymatic degradation of crude oil was accompanied by dynamic production of gases (CO2 and H-2) and organic acids (oxalate and propionate). In addition, the enzyme preparations removed 83.40-87.78% of crude oil adsorbed on filter paper. @@@ CONCLUSIONExtracellular enzymes from Aspergillus spp. are efficient in degrading crude oil, and have great potential for applications in enhanced crude oil recovery.
