A novel model of distal colon cancer in athymic mice

作者：Priolli Denise Goncalves^*; Abrantes Ana Margarida; Neves Silvia; Batista Joana Neves; Cardinalli Izilda Aparecida; Botelho Maria Filomena

来源：Acta Cirurgica Brasileira, 2012, 27(6): 355-360.

DOI：10.1590/S0102-86502012000600001

• Xenograft Model Antitumor Assays, Colorectal Neoplasms
• Radionuclide Imaging
• Technetium Tc 99m Sestamibi
• Mice, Nude

PURPOSE: The present a novel adenocarcinoma model in athymic mice. %26lt;br%26gt;METHODS: Seven athymic mice were used. Colon diversion and distal fistula were made. Adenocarcinoma cells were inoculated in the submucosa of fistula. Tumor growth was monitored daily. Scintigraphy with 99mTc-MIBI was performed to identify the tumor. %26lt;br%26gt;RESULTS: The model of distal colon cancer is feasible. Tumor detection was possible by both, macroscopically and molecular imaging. All resections demonstrated poorly differentiated tumors. Colon obstruction occurred in one case, similarly to evolution in human tumors of distal colon. %26lt;br%26gt;CONCLUSION: The proposed model of distal colon cancer is feasible, allows for easy monitoring of tumoral growth by both, macroscopically and molecular imaging, and is suitable for studying the evolution of tumor with implementation of cytotoxic therapy in vivo.

• 出版日期2012-6