Molecular and immunohistochemical diagnosis of Francisella noatunensis subsp orientalis from formalin-fixed, paraffin-embedded tissues

作者：Soto Esteban^*; Illanes Oscar; Hilchie David; Morales Juan A; Sunyakumthorn Piyanate; Hawke John P; Goodwin Andrew E; Riggs Allen; Yanong Roy P; Pouder Deborah B; Francis Floyd Ruth; Arauz Maziel; Bogdanovic Lewis; Castillo Alcala Fernanda

来源：Journal of Veterinary Diagnostic Investigation, 2012, 24(5): 840-845.

DOI：10.1177/1040638712452108

• Francisella noatunensis
• iglC
• immunohistochemistry
• real-time polymerase chain reaction

Members of the genus Francisella (viz., F. noatunensis subsp. orientalis [Fno] and F. noatunensis subsp. noatunensis) have been described as causative agents of chronic granulomatous and pyogranulomatous lesions in wild and cultured fish species. In the present study, 68 archived formalin-fixed, paraffin-embedded (FFPE) tissues from several fish species, collected at different geographical locations from 2000 to 2011, were analyzed using a real-time polymerase chain reaction assay for the detection of the Fno intracellular growth loci C (iglC) gene and by immunohistochemistry for the demonstration of Fno antigens. The results revealed a high correlation between these 2 diagnostic techniques validating their use for the diagnosis of Fno infection in archived FFPE tissues and confirming the presence of Fno in fish species from the Caribbean, Hawaii, and continental North and South America in the early years of the present century.

• 出版日期2012-9