A reversible gene trap collection empowers haploid genetics in human cells

作者：Buerckstuemmer Tilmann; Banning Carina; Hainzl Philipp; Schobe**erger Richard; Kerzendorfer Claudia; Pauler Florian M; Chen Doris; Them Nicole; Schischlik Fiorella; Rebsamen Manuele; Smida Michal; de la Cruz Ferran Fece; Lapao Ana; Liszt Melissa; Eizinger Benjamin; Guenzl Philipp M; Blomen Vincent A; Konopka Tomasz; Gapp Bianca; Parapatics Katja; Maier Barbara; Stoeckl Johannes; Fischl Wolfgang; Salic Sejla; Casari M Rita Taba; Knapp Sylvia; Bennett Keiryn L

来源：Nature Methods, 2013, 10(10): 965-+.

DOI：10.1038/nmeth.2609

Knockout collections are invaluable tools for studying model organisms such as yeast. However, there are no largescale knockout collections of human cells. Using gene-trap mutagenesis in near-haploid human cells, we established a platform to generate and isolate individual %26apos;gene-trapped cells%26apos; and used it to prepare a collection of human cell lines carrying single gene-trap insertions. In most cases, the insertion can be reversed. This growing library covers 3,396 genes, one-third of the expressed genome, is DNA-barcoded and allows systematic screens for a wide variety of cellular phenotypes. We examined cellular responses to TNF-alpha, TGF-beta, IFN-gamma and TNF-related apoptosis-inducing ligand (TRAIL), to illustrate the value of this unique collection of isogenic human cell lines.

• 出版日期2013-10