A label-free and sensitive fluorescent method for the detection of uracil-DNA glycosylase activity

作者:Tao Jing; Song Panshu; Sato Yusuke; Nishizawa Seiichi; Teramae Norio; Tong Aijun; Xiang Yu*
来源:Chemical Communications, 2015, 51(5): 929-932.
DOI:10.1039/c4cc06170e

摘要

The activity of uracil-DNA glycosylase (UDG), an enzyme in the base excision repair, is detected at a high sensitivity by a DNA substrate containing only one uracil through a label-free fluorescent approach, which is also successfully applied for the measurement of UDG inhibitors.