Ameliorative response of some essential oil furanocoumarins and proteins from Psoralea corylifolia against gamma-irradiation induced oxidative stress

作者：Bhat Towseef Mohsin^*; Choudhary Sana; Ansari M Y K; Aslam Rumana; Dar Sabzar Ahmed

来源：Industrial Crops and Products, 2015, 76: 422-431.

DOI：10.1016/j.indcrop.2015.06.059

• P. corylifolia
• Gamma radiation
• Psoralen
• MALDI-TOF-MS
• Protein
• GC-MS

Secondary metabolism producing essential oil furanocoumarins depends on various environmental factors and ionizing radiation is currently a very important way of enhancing these compounds through radio stimulation. The study aims to evaluate the ameliorative effect of essential oil furanocoumarins and proteins from Psoralea corylifolia against gamma-irradiation-induced oxidative stress. Antioxidant biomarkers like H2O2, peroxidase, superoxide dismutase, and catalase increased significantly (p < 0.01) in all treated groups. Dose dependent increase of H2O2 concentration leads to significant chromosomal abnormalities. Essential oil (EO) yield and furanocoumarin content showed a significant (p < 0.01) increase up to 200 Gy. These contents decreased significantly on increased expression of proteins involved in furanocoumarin biosynthesis and stress defense. DPHH and Phosphomolybdenum assays showed a significant (p < 0.01) antioxidant activity in the essential oil on varying doses of gamma radiation. It is concluded that mild doses of gamma irradiation is an important abiotic-elicitor for enhancing the essential oil yield and furanocoumarin content in P. corylifolia which possess important radio resistant properties.

• 出版日期2015-12-15