Alpha-eleostearic acid suppresses proliferation of MCF-7 breast cancer cells via activation of PPAR gamma and inhibition of ERK 1/2

作者：Moon Hyun Seuk; Guo Ding Ding; Lee Hong Gu; Choi Yun Jaie; Kang Jae Seong; Jo Kyungmin; Eom Jung Min; Yun Cheol Heui^*; Cho Chong Su

来源：Cancer Science, 2010, 101(2): 396-402.

DOI：10.1111/j.1349-7006.2009.01389.x

Alpha-eleostearic acid (alpha-ESA) is known to suppress the growth in cancer cells although its underlying molecular mechanisms have not been fully elucidated. The present study was designed to elucidate and evaluate the anticancer mechanism of alpha-ESA on MCF-7 breast cancer cells. Also, an attempt was made to better understand the anticancer mechanism by which alpha-ESA activated PPAR gamma and attenuated the ERK1/2 MAPK phosphorylation state. The MCF-7 breast cancer cell-line and nontumorigenic MCF-10A human mammary epithelial cells were treated with alpha-ESA and compared with negative control (without treatment) and positive control groups (treated with rosiglitazone), and changes of apoptosis-related molecules, PPAR gamma and pERK1/2 were examined. In MCF-7 cells treated with alpha-ESA, we found that the expression of p53, p21, and Bax was up-regulated whereas expression of Bcl-2 and procaspase-9 was down-regulated. Moreover, nuclear translocation of PPAR gamma by alpha-ESA positively correlated with inhibition of ERK1/2 activation. Our data suggest that alpha-ESA can be considered to be a PPAR gamma agonist and thus a candidate for a chemotherapeutic agent against breast cancer. (Cancer Sci 2010; 101: 396-402)

• 出版日期2010-2