A new human IgG avidity test, using mixtures of recombinant antigens (rROP1, rSAG2, rGRA6), for the diagnosis of difficult-to-identify phases of toxoplasmosis

作者：Drapala Dorota; Holec Gasior Lucyna^*; Kur Jozef; Ferra Bartlomiej; Hiszczynska Sawicka Elzbieta; Lautenbach Dariusz

来源：Diagnostic Microbiology and Infectious Disease, 2014, 79(3): 342-346.

DOI：10.1016/j.diagmicrobio.2014.03.005

• Toxoplasmosis
• IgG avidity
• Recombinant antigens
• ROP1
• SAG2
• GRA6

The preliminary diagnostic utility of two mixtures of Toxoplasma gondii recombinant antigens (rROP1 + rSAG2 and rROP1 +rGRA6) in IgG ELISA and IgG avidity test has been evaluated. A total of 173 serum samples from patients with toxoplasmosis and seronegative people were examined. The sensitivity of IgG ELISA for rROP1 +rSAG2 and rROPl+rGRA6 was 91.1% and 76.7%, respectively, while the reactivity for sera from patients where acute toxoplasmosis was suspected was higher, at 100% and 95.4%, respectively, than for people with chronic infection, at 88.2% and 70.6%. In this study a different trend in avidity maturation of IgG antibodies for two mixtures of proteins in comparison with native antigen was observed. The results suggest that a new IgG avidity test using the mixtures of recombinant antigens may be useful for the diagnosis of difficult-to-identify phases of toxoplasmosis. For this reason, selected mixtures after the additional tests on groups of sera with well-defined dates of infection could be used as a better alternative to the native antigens of the parasite in the serodiagnosis of human T. gondii infection.

• 出版日期2014-7