A study of the effect on human mesenchymal stem cells of an atmospheric pressure plasma source driven by different voltage waveforms

作者：Laurita R^*; Alviano F; Marchionni C; Abruzzo P M; Bolotta A; Bonsi L; Colombo V; Gherardi M; Liguori A; Ricci F; Rossi M; Stancampiano A; Tazzari P L; Marini M

来源：Journal of Physics D: Applied Physics , 2016, 49(36): 364003.

DOI：10.1088/0022-3727/49/36/364003

• atmospheric pressure non-equilibrium plasma
• dielectric barrier discharge
• high voltage pulsed discharge
• fetal membrane-derived human mesenchymal stem cells

The effect of an atmospheric pressure non-equilibrium plasma on human mesenchymal stem cells was investigated. A dielectric barrier discharge non-equilibrium plasma source driven by two different high-voltage pulsed generators was used and cell survival, senescence, proliferation, and differentiation were evaluated. Cells deprived of the culture medium and treated with nanosecond pulsed plasma showed a higher mortality rate, while higher survival and retention of proliferation were observed in cells treated with microsecond pulsed plasma in the presence of the culture medium. While a few treated cells showed the hallmarks of senescence, unexpected delayed apoptosis ensued in cells exposed to plasma-treated medium. The plasma treatment did not change the expression of OCT4, a marker of mesenchymal stem cell differentiation.

• 出版日期2016-9-14