Dy3+ and Nd3+ induced genetic mutation of bacillus alpha-amylase

作者：Zhang Dongyan^*; Hu Jianhua; Siqin Bater; Zhang Tong; Chang Ying

来源：Journal of Inorganic Biochemistry, 2009, 103(7): 935-939.

DOI：10.1016/j.jinorgbio.2008.12.002

• Rare-earth
• amyE
• Mutation

After treatment with DyCl3 and NdCl3, two strains of mutated bacilli showing increased alpha-amylase activity were isolated. The alpha-amylase genes were amplified, cloned, and sequenced. Sequencing revealed that there were either 11 or 14 bases altered in the two genes. These alterations took the form of base sub-stitutions, and transversion was more common than transition. Based oil these results, it was concluded that the rare earth compounds DyCl3 and NdCl3 were mutagens.

• 出版日期2009-7
• 单位内蒙古工业大学