Assembly and annotation of full mitochondrial genomes for the corn rootworm species, Diabrotica virgifera virgifera and Diabrotica barberi (Insecta: Coleoptera: Chrysomelidae), using Next Generation Sequence data

作者：Coates Brad S^*

来源：Gene, 2014, 542(2): 190-197.

DOI：10.1016/j.gene.2014.03.035

• Next Generation Sequencing
• Full mitochondrial genome
• Molecular divergence
• Phylogenetics

Complete mitochondrial genomes for two corn rootworm species, Diabrotica virgifera virgifera (16,747 bp) and Diabrotica barberi (16,632; Insecta: Coleoptera: Chrysomelidae), were assembled from Illumina HiSeq2000 read data. Annotation indicated that the order and orientation of 13 protein coding genes (PCGs), and,22 tRNA and 2 rRNA sequences were in typical of insect mitochondrial genomes. Non-standard nad4 and cox3 stop codons were composed of single T nucleotides and likely completed by adenylation, and atypical ITT start codons was predicted for both D. v. virgtfera and D. barberi nad1 genes. The D. v. vireera and D. barberi haplotypes showed 819 variable nucleotide positions within PCG regions (7.36% divergence), which suggest that speciation may have occurred similar to 3.68 million years ago assuming a linear rate of short-term substitution. Phylogenetic analyses of Coleopteran MID genome show clustering based on family level, and may have the capacity to resolve the evolutionary history within this Order of insects. Published by Elsevier B.V.

• 出版日期2014-6-1