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摘要
Pest-resistant strains of Pinus massoniana Lamb. can defend themselves against pests. However, the mechanisms underlying pest resistance in them remain. Next-generation sequencing (NGS) technology was employed to examine the transcriptomes of insect-resistant and insect-sensitive strains of P. massoniana. In this investigation 98,547 transcripts and 55,824 unigenes were obtained. Among the unigenes, 35,214 sequences (63.08%) were annotated. Gene expression levels in strains with various levels of insect resistance were compared. After this comparison 511 genes were found to be differentially expressed. Pathway analysis of the differentially expressed genes revealed a number of important pathways, including the "terpenoid metabolic pathway" and the "flavonoid metabolic pathway". The present study revealed that several important transcription factors (TFs), including WRKY and the Apetala2/ethylene responsive factor (AP2/ERF), as well as important enzymes such as abietadiene synthase, play significant roles in anti-pest responses. Discovery of these defense genes and pathways is conducive to further understanding of the mechanisms by which P. massoniana defends itself against pests. In addition, 11 genes that were assumed to participate in anti-pest responses were examined by real-time quantitative PCR to verify the accuracy of the transcriptome data. This study identified the metabolic pathways and related genes expressed by insect-resistant P. massoniana varieties to improve their ability to defend against pests. The above data will provide multidimensional information regarding the molecular mechanisms of insect resistance in PM for the future researchers.
- 出版日期2016-9
- 单位广西壮族自治区林业科学研究院
