An alternative method of promoter assessment by confocal laser scanning microscopy

作者：Sahoo Dipak K; Ranjan Rajiv; Kumar Deepak; Kumar Alok; Sahoo Bhabani S; Raha Sumita; Maiti Indu B; Dey Nrisingha^*

来源：Journal of Virological Methods, 2009, 161(1): 114-121.

DOI：10.1016/j.jviromet.2009.06.011

• Confocal laser scanning microscopy
• Green fluorescent protein
• beta-Glucuronidase
• Pararetrovirus promoter
• Promoter activity

A rapid and useful method of promoter activity analysis using techniques of confocal laser scanning microscopy (CLSM) is described in the present study. The activities of some pararetroviral promoters such as CaMV35S (Cauliflower mosaic virus), FMVSgt3 (Figwort mosaic virus sub-genomic transcript) and MMVFLt12 (Mirabilis mosaic virus full-length transcript) coupled to GFP (green fluorescent protein) and GUS (beta-glucuronidase) reporter genes were determined simultaneously by the CLSM technique and other available conventional methods for reporter gene assay based on relevant biochemical and molecular approaches. Consistent and comparable results obtained by CLSM as well as by other conventional assay methods confirm the effectiveness of the CLSM approach for assessment of promoter activity. Hence the CLSM method can be suggested as an alternative way for promoter analysis on the basis of high throughput.

• 出版日期2009-10