科研之友
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摘要
With the realization of the growing popularity and demand for phytopharmaceuticals, standardization is becoming a mandatory part of the regulation of the herbal drug industry. This is applicable both to humans and veterinary formulations. Stresroak premix is a renowned polyherbal formulation for poultry. It is used as an immunomodulator, an antistress treatment, an adaptogenic, and a performance enhancer. It is a blend of Phyllanthus emblica L. (Euphorbiaceae), Withania somnifera L. (Solanacaeae), Mangifera indica L. (Anacardiaceae), Ocimum sanctum L. (Lamiaceae), and Shilajit. An HPTLC method has been established for standardization and quantification of gallic acid, mangiferin, and withanolide A in Stresroak premix. The average content of these markers in different batches of the formulation was 0.654, 0.627, and 0.325% (w/w), respectively. The method was validated for instrumental precision, repeatability, and accuracy. Average recovery of gallic acid, mangiferin, and withanolide A was 100.41, 99.29, and 98.04% (w/w), respectively. The method is simple, precise, specific, and accurate and has potential for use in the routine quality control of the formulation and its raw materials.
- 出版日期2011-2
