A Yeast Bioassay for Androgenic and Anti-Androgenic Compounds Based on the NH2- and COOH-Terminal Interaction of Androgen Receptor

作者：Ogawa Masahiro; Yamaji Ryoichi^*; Mitani Takakazu; Murata Yohei; Nakao Motoyuki; Harada Naoki; Nakano Yoshihisa; Inui Hiroshi

来源：Bioscience Biotechnology and Biochemistry, 2010, 74(9): 1965-1968.

DOI：10.1271/bbb.100323

• androgen receptor (AR)
• bioassay
• N-C interaction
• beta-catenin
• yeast two-hybrid

Androgenic compounds induce an interaction between the NH2- and COOH-terminal regions (N-C interaction) of androgen receptor (AR). We describe a rapid yeast bioassay for androgenic and anti-androgenic compounds based on androgen-dependent beta-catenin-enhanced N-C interaction. The bioassay was also effective at detecting compounds that inhibit the N-C interaction in ways that do not involve binding to the ligand-binding domain.

• 出版日期2010-9