NANOSECOND LASER HEATING OF GOLD NANOPARTICLES. APPLICATION IN PHOTOTHERMAL CANCER CELL THERAPY

作者：Nedyalkov Nikolay N^*; Imamova Semra E; Atanasov Petar A; Toshkova Reneta A; Gardeva Elena G; Yossifova Liliya S; Alexandrov Marin T

来源：Comptes Rendus de l Academie Bulgare des Sciences, 2010, 63(5): 767-774.

• gold nanoparticles
• photothermal therapy of cancer cells

In this paper theoretical and experimental results on the heating process of gold nanoparticles irradiated by nanosecond laser pulses are presented. Gold nanoparticles with diameters of 40 and 100 nm in water are considered in the presented study. Based on the Mie scattering theory the optimal conditions of particle absorption for different wavelengths in the visible spectra are estimated. The calculated absorption cross-sections are implemented in 2D heat diffusion equation which describes the particle and the surrounding medium heating process. Using this model the dependence of the achieved maximal particles temperature on the applied laser fluence is obtained. The results indicate that even at laser fluences lower by order of magnitude than the typical threshold for tissue modification, the gold nanoparticles are heated to temperatures higher than the critical for water. Such temperatures are sufficient to induce permanent modification in a biological object in a close vicinity of the particle. The efficiency of this process is estimated by performing in vitro photothermal therapy of HeLa cancer cells, mediated by gold nanoparticles with diameter of 40 and 100 nm. The strongest cytotoxic effect is observed in the case of application of gold nanoparticles with diameters of 100 nm.

• 出版日期2010