An UDP-Glucosyltransferase Gene from Barley Confers Disease Resistance to Fusarium Head Blight

作者：Xing, Li-Ping; He, Li-Qiang; Xiao, Jin; Chen, Qi-Guang; Li, Ming-Hao; Shang, Yi; Zhu, Yan-Fei; Chen, Pei-Du; Cao, Ai-Zhong^*; Wang, Xiu-E^*

来源：Plant Molecular Biology Reporter, 2017, 35(2): 224-236.

DOI：10.1007/s11105-016-1014-y

• UDP-glucosyltransferase gene
• Hordeum vulgare L
• Fusarium head blight (FHB)
• Deoxynivalenol (DON)
• In vitro antifungal activity
• Virus-induced gene silencing (VIGS)

UDP-glucosyltransferases (UGTs) contribute to Fusarium head blight (FHB) resistance of wheat and barley by glycosylating the deoxynivalenol (DON), which is produced by Fusarium fungus. In this study, seven alleles of barley HvUGT14077 (GenBank No.GU170356.1) were cloned using RT-PCR. Among them, HvUGT-10W1, which was isolated from a FHB resistant barley variety 10W1, was significantly up-regulated in young spikes after F. graminearum (F.g) inoculation. HvUGT-10W1::GFP was subcellularly located in the plasma membrane and cytoplasm of the wheat protoplasts. In vitro antifungal activity assay showed that the HvUGT-10W1 protein exerted obvious inhibition against the growth of F.g. The silencing of the HvUGT-10W1 by virus-induced gene silencing (VIGS) resulted in compromised FHB resistance of 10W1, which was shown by the increased infected colonies on the leaves. These indicated that the barley HvUGT-10W1 may also contribute to F.g resistance in barley and provided a potential candidate gene to develop transgenic barley with enhanced FHB resistance.

• 出版日期2017-4
• 单位作物遗传与种质创新国家重点实验室; 中国科学院; 浙江省农业科学院; 中国科学院合肥物质科学研究院; 南京农业大学