Analysis of Ion Transport through a Single Channel of Gramicidin A in Bilayer Lipid Membranes

作者：Kubota Shintaro; Shirai Osamu^*; Kitazumi Yuki; Kano Kenji

来源：Analytical Sciences, 2016, 32(2): 189-192.

DOI：10.2116/analsci.32.189

• Gramicidin A
• bilayer lipid membrane
• ion transport
• channel
• permselectivity
• antiport
• voltammetry

Ion transport through a single channel of gramicidin A (GA) within the bilayer lipid membrane (BLM) between two aqueous phases (W1 and W2) has been analyzed based on the electroneutrality principle. The single-channel current increases in proportion to the magnitude of the applied membrane potential and is also dependent on the permeability coefficients of electrolyte ions (K+ and Cl-). By varying the ratio of the concentration of KCl in W1 to that in W2, the ratio of the diffusion coefficient of K+ in the BLM to that of Cl- in the BLM can be evaluated.

• 出版日期2016-2