科研之友
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摘要
The determination of cancer biomarkers plays an important role in early cancer screening and disease diagnosis. Herein, we designed a sandwiched "signal on" electrochemiluminescence (ECL) immunosensor to alpha fetoprotein (AFP) using gold nanoparticle-modified graphite-like carbon nitride nanosheets (Au NPs@ g-C3N4 NSs) nanocomposite as the signal tag. The capture probes of primary antibodies were immobilized on the surface of the modified electrode of MSAPANI/MWCNTs/GCE with high electrical conductivity. Where MSA, PANI, MWCTs, and GCE are mercaptosuccinic acid, polyaniline and multiwalled carbon nanotubes, and glass carbon electrode, respectively. The amount of AFP was quantified by the cathodic ECL of g-C3N4 NSs from the sandwich type immunocomplex. Under the optimized conditions, the proposed sensing strategy provides a measurable AFP concentration range from 0.1 pg mL(-1) to 1 ng mL(-1) with a limit of detection of 0.03 pg mL(-1). Such protocol exhibits high sensitivity, good stability and promising potential applications in clinical analysis.
