A technique for repeated recordings in cortical organotypic slices

作者：Dong HW; Buonomano DV^*

来源：Journal of Neuroscience Methods, 2005, 146(1): 69-75.

DOI：10.1016/j.jneumeth.2005.01.017

• organotypic
• long-term
• repeated recordings
• biocytin
• input-output
• cortex

Electrophysiology studies in vitro have generally focused on forms of plasticity which are rapidly induced and last for minutes to hours. However, it is well known that plasticity at some cellular and synaptic loci are induced and expressed over many hours or days. One limitation in examining these forms of plasticity is the lack of preparations that allow stimulation and recording of the same tissue over a 24 h period or more. Here we describe a simple method for repeated recordings and stimulating the same organotypic slices (different neurons) over a 24 h We use the conventional interface organotypic culture method together with a custom chamber, which allows recordings on the intact filter, and DiI to mark the stimulation sites. We show that the health of the neurons, as defined by intrinsic excitability, excitatory and inhibitory input-output curves, and morphology remains unchanged over the 24 h period. This simple technique provides a means to investigate long-term forms of plasticity that may be induced under conditions similar to those observed in vivo. Additionally, it provides the opportunity to perform long-term morphological and pharmacological studies.

• 出版日期2005-7-15