Antimicrobial peptide protonectin disturbs the membrane integrity and induces ROS production in yeast cells

作者：Wang Kairong; Dang Wen; Xie Junqiu; Zhu Ranran; Sun Mengyang; Jia Fengjing; Zhao Yanyan; An Xiaoping; Qiu Shuai; Li Xiaoyuan; Ma Zelin; Yan Wenjin^*; Wang Rui

来源：Biochimica et Biophysica Acta-Biomembranes, 2015, 1848(10): 2365-2373.

DOI：10.1016/j.bbamem.2015.07.008

• Protonectin
• Antifungal
• ROS production
• Biofilm formation

Candidiasis is often observed in immunocompromised patients and is the 4th most common cause of bloodstream infections. However, antifungals are limited, so novel antifungal agents are urgently needed. Antimicrobial peptides (AMPs) are considered as potential alternatives of conventional antibiotics. In the present study, antimicrobial peptide protonectin was chemically synthesized and its antifungal activity and mode of action were studied. Our results showed that protonectin has potent antifungal activity and fungicidal activity against the tested fungi cells. Its action mode involved the disruption of the membrane integrity and the inducing of the production of cellular ROS. Furthermore, protonectin could inhibit the formation of biofilm and kill the adherent fungi cells. In conclusion, with the increase of fungal infection, protonectin may offer a new strategy and be considered as a potential therapeutic agent against fungal disease.

• 出版日期2015-10
• 单位兰州大学