Analysis of process and mechanism of bioclogging in aqueous media

作者：Xia Lu; Zheng Xi Lai^*; Duan Yu Huan; Peng Tao

来源：Journal of Hydraulic Engineering, 2014, 45(6): 749-755.

DOI：10.13243/j.cnki.slxb.2014.06.015

• Aqueous media
• Bio-clogging
• Biological technology
• DGGE
• Extra-cellular polymeric substances
• Methylobacterium
• Microbial growth
• PCR

Sand samples collecting from the Dagu River in Qingdao city were used as representative aquifer medium, and measured the composition and properties. Percolating experiments were conducted to investigate the dynamic changes of different flow systems. The molecular biological technology PCR-DGGE was used to identify the advantage microorganisms inducing bioclogging. In addition, the use of nutrients and oxygen by microbial were analysis in order to discuss the bioclogging process and its mechanism. The results indicate that the degree of bioclogging in aquifer media decreases as the percolating distances increase and the permeability of aquifer medium presents obvious heterogeneity. After a short adjusting period, microbial quickly enters a growth stage. Meanwhile, microbial clogging in aquifer media develops fast. The main microorganism which causing bioclogging are Methylobacterium, Janthinobacterium, Yersinia, Staphylococcus as well as Acidovorax. In particular, Methylobacterium, Janthinobacterium, Staphylococcus and Acidovorax are Myxobacterium. It is proved that there are microbial reproduction, metabolism as well as other activities in aquifer media. High nutrient loading provides ample oxygen for microbial growth and promotes production of large amounts of extracellular polymeric substances which caused the most bioclogging in media.

• 出版日期2014