Antiphospholipid Antibodies in Dogs with Immune Mediated Hemolytic Anemia, Spontaneous Thrombosis, and Hyperadrenocorticism

作者：Miller A G; Dow S; Long L; Olver C S^*

来源：Journal of Veterinary Internal Medicine, 2012, 26(3): 614-623.

DOI：10.1111/j.1939-1676.2012.00922.x

• Anemia
• Antiphospholipid syndrome
• Beta-2 glycoprotein 1
• Lupus anticoagulant
• Thromboembolism

Background The pathophysiology of thrombus formation in canine IMHA and other diseases remains unclear. Antiphospholipid antibodies (aPL) are an important cause of thrombosis in humans and might cause thrombosis in dogs. Hypothesis Dogs with IMHA, spontaneous thrombosis, and hyperadrenocorticism will have increased levels of aPL and lupus anticoagulants (LA), compared with healthy and sick dogs. Animals Thre aPL were measured in healthy controls (n similar to 4045); sick dogs without thrombosis (n similar to 86); IMHA (n similar to 37); spontaneous thrombosis (ST, n similar to 11); and hyperadrenocorticism (n similar to 17). Four groups of dogs were also tested for the presence of LA: healthy controls (n similar to 40); sick dogs without thrombosis (n similar to 13); IMHA (n similar to 13); and ST (n similar to 5). Methods Prospective cohort study. Dogs were tested for aPL by an ELISA and for LA by the dilute Russell%26apos;s Viper venom time (dRVVT). Median values were compared by KruskalWallis (aPL) or ANOVA (LA), and an odds ratio for development of thrombosis in dogs positive for aPL was calculated. Results aPL are uncommon in healthy dogs. A total of 13/86 sick dogs without thrombosis, 7/37 dogs with IMHA, 1/11 dogs with ST, and 3/17 dogs with HAC were positive for protein binding-dependent aPL. There was no significant difference in the number of dogs positive for aPL for any of the study groups, and there was no increased risk for thrombosis in dogs positive for aPL. No dogs had LA. Conclusions Our preliminary research does not support a strong role for aPL for the development of thrombosis in dogs with IMHA and other thombotic diseases, although future studies are warranted.

• 出版日期2012-6