The production of 1,3-propanediol from mixtures of glycerol and glucose by a Klebsiella pneumoniae mutant deficient in carbon catabolite repression

作者：Oh Baek Rock; Hong Won Kyung; Heo Sun Yeon; Luo Lian Hua; Kondo Akihiko; Seo Jeong Woo^*; Kim Chul Ho

来源：Bioresource Technology, 2013, 130: 719-724.

DOI：10.1016/j.biortech.2012.12.076

• Klebsiella pneumoniae
• Carbon catabolite repression
• crr
• 1,3-Propanediol
• Mixtures of glycerol and glucose

In the present study, mutant strain of Klebsiella pneumoniae with deletion of the crr gene encoding EIIA(Glc) (a component of the glucose-specific phosphoenolpyruvate-dependent transferase system [PTS]) was prepared. This eliminated the ability of the strain to mediate carbon catabolite repression (CCR). Production of 1,3-propanediol (1,3-PD) from glycerol by the crr mutant strain was enhanced (compared to that of the parent) in the presence of glucose. Using molasses as a co-substrate of glycerol, the maximum yield of 1,3-PD was 60.4% greater (81.2 g/l) than that obtained when glycerol was used alone, under optimum fermentation conditions.

• 出版日期2013-2