科研之友
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摘要
Tamoxifen is the first line chemotherapy reagent in estrogen receptor positive breast cancer treatment. Here we examined the role of arsenic trioxide in proliferation, migration and invasion of breast cancer cells in combination with tamoxifen. And the relative molecular mechanism was described. The proliferation of breast cancer cells was assessed by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay after treatment with arsenic trioxide. The effect of arsenic trioxide on tamoxifen-induced apoptosis of breast cancer cells was evaluated by Caspase-Glo3/7 assay. The migration and invasion were examined by BD Transwell migration and matrigel invasion assays. The apoptotic proteins were detected by western blot. We found arsenic trioxide enhanced the inhibitory effect of tamoxifen on the proliferation, migration and invasion of breast cancer cells. Arsenic trioxide increased the radiation-induced apoptosis. The expression of AKT was decreased, and the PTEN expression level was increased in breast cancer cells after treatment with tamoxifen and arsenic trioxide. Our study indicates that arsenic trioxide plays critical roles in breast cancer treatment in combination with tamoxifen.
- 出版日期2015
- 单位长春中医药大学
