Novel approaches: Tissue engineering and stem cells - In vitro modelling of the gut

作者：van Rijn Jorik M; Schneeberger Kerstin; Wiegerinck Caroline L; Nieuwenhuis Edward E S; Middendorp Sabine^*

来源：Best Practice & Research in Clinical Gastroenterology, 2016, 30(2): 281-293.

DOI：10.1016/j.bpg.2016.03.005

• Organoids
• Intestinal epithelial cells
• Disease modelling
• Stem cell therapy

In many intestinal diseases, the function of the epithelial lining is impaired. In this review, we describe the recent developments of in vitro intestinal stem cell cultures. When these stem cells are grown in 3D structures (organoids), they provide a model of the intestinal epithelium, which is closely similar to the growth and development of the in vivo gut. This model provides a new tool to study various diseases of malabsorption in functional detail and therapeutic applications, which could not be achieved with traditional cell lines. First, we describe the organization and function of the healthy small intestinal epithelium. Then, we discuss the establishment of organoid cultures and how these structures represent the healthy epithelium. Finally, we discuss organoid cultures as a tool for studying intrinsic properties of the epithelium, as a model for intestinal disease, and as a possible source for stem cell transplantations.

• 出版日期2016-4