A novel glutathione-S transferase immunosensor based on horseradish peroxidase and double-layer gold nanoparticles

作者：Lu, Dingqiang; Lu, Fuping; Pang, Guangchang^*

来源：Biomedical Microdevices, 2016, 18(3): 50.

DOI：10.1007/s10544-016-0075-x

• GST
• Electrochemical immunosensor
• The amperometric I-t curve method
• GNPs

GSTs, a biotransformation enzyme group, can perform metabolism, drug transfer and detoxification functions. Rapid detection of the GSTs with more sensitive approaches is of great importance. In the current study, a novel double-layer gold nanoparticles-electrochemical immunosensor electrode (DGN-EIE) immobilized with Glutathione S-Transferase (GST) antibody derived from Balb/c mice was developed. To increase the fixed quantity of antibodies and electrochemical signal, an electrochemical biosensing signal amplification system was utilized with gold nanoparticles-thionine-chitosan absorbing horseradish peroxidase (HRP). In addition, transmission electron microscope (TEM) was used to characterize the nanogold solution. To evaluate the quality of DGN-EIE, the amperometric I-t curve method was applied to determine the GST in PBS. The results showed that the response current had a good linear correlation with the GST concentration ranged from 0.1-10(4) pg/mL. The lowest detection limit was found at 0.03 pg/mL(S/N = 3). The linear equation was deduced as Delta I/% = 7.386lgC + 22.36 (R-2 = 0.998). Moreover, it was validated with high sensitivity and reproducibility. Apparently, DGN-EIE may be a very useful tool for monitoring the GST.

• 出版日期2016-6
• 单位天津科技大学; 天津商业大学