Ag-NPs induce apoptosis, mitochondrial damages and MT3/OSGIN2 expression changes in an in vitro model of human dental-pulp-stem-cells-derived neurons

作者：Bonaventura Gabriele; La Cognata Valentina; Lemmolo Rosario; Zimbone Massimo; Contino Annalinda; Maccarrone Giuseppe; Failla Bruno; Barcellona Maria Luisa; Conforti Francesca Luisa; D'Agata Velia; Cavallaro Sebastiano^*

来源：NeuroToxicology, 2018, 67: 84-93.

DOI：10.1016/j.neuro.2018.04.014

• Dental pulp stem cells
• Neurons
• Silver nanoparticles
• Toxicity

Silver nanoparticles (Ag-NPs) are one of the most popular nanotechnologies because of their unique antibacterial and antifungal properties. Given their increasing use in a wide range of commercial, biomedical and food products, exposure to Ag-NPs is now a reality in people's lives. However, there is a serious lack of information regarding their potential toxic effects in the central nervous system. In this study, we investigated the biocompatibility of "homemade" Ag-NPs in an in vitro model of human neurons derived from dental pulp mesenchymal stem cells. Our results showed that acute exposure to Ag-NPs cause cytotoxicity, by triggering cell apoptosis, damaging neuronal connections, affecting the mitochondrial activity and changing the mRNA expression level of MT3 and OSGIN2, two genes involved in heavy metals metabolism and cellular growth during oxidative stress conditions. Further studies are needed to understand the molecular mechanisms and the physiological consequences underlying Ag-NPs exposure.

• 出版日期2018-7