Apoptin induces apoptosis in an oral cancer mouse model

作者：Schoop Remilio A L^*; de Jong Robert J Baatenburg; Noteborn Mathieu H M

来源：Cancer Biology and Therapy, 2008, 7(9): 1370-1375.

• apoptin
• apoptosis
• caspase-3
• immunocompetent mice
• recombinant adenovirus
• head and neck squamous cell carcinoma
• 4-Nitroquinoline-1-oxide

Apoptin, a chicken anemia virus-derived protein, induces apoptosis in various tumor cell lines and xenografted tumors. Its apoptotic activity is not hampered by tumor-suppressor p53 mutations or overexpression of anti-apoptosis proteins Bcl-2 or Bcl-xL. We report for the first time the effects of apoptin expression in primary oral tumors, induced by the carcinogen 4-Nitroquinoline1-oxide in immunocompetent mice. In vivo a significant amount of primary oral tumor cells expressing apoptin cells underwent apoptosis, whereas synthesis of the LacZ control product did not. Ectopical expression of apoptin in passage 1 cell cultures derived from these oral tumors also resulted in apoptin-induced. Both in-vivo and in-vitro treated cells underwent apoptosis via the activation of caspase-3. The fact that apoptin induces apoptosis in primary squamous cell carcinoma cells indicates that apoptin is a potential therapeutic agent for treatment of head and neck squamous cell carcinoma.

• 出版日期2008-9