A Krebs Cycle Component Limits Caspase Activation Rate through Mitochondrial Surface Restriction of CRL Activation

作者：Aram Lior; Braun Tslil; Braverman Carmel; Kaplan Yosef; Ravid Liat; Levin Zaidman Smadar; Arama Eli^*

来源：Developmental Cell, 2016, 37(1): 15-33.

DOI：10.1016/j.devcel.2016.02.025

How cells avoid excessive caspase activity and unwanted cell death during apoptotic caspase-mediated removal of large cellular structures is poorly understood. We investigate caspase-mediated extrusion of spermatid cytoplasmic contents in Drosophila during spermatid individualization. We show that a Krebs cycle component, the ATP-specific form of the succinyl-CoA synthetase beta subunit (A-Sb), binds to and activates the Cullin-3-based ubiquitin ligase (CRL3) complex required for caspase activation in spermatids. In vitro and in vivo evidence suggests that this interaction occurs on the mitochondrial surface, thereby limiting the source of CRL3 complex activation to the vicinity of this organelle and reducing the potential rate of caspase activation by at least 60%. Domain swapping between A-Sb and the GTP-specific SCS beta (G-S beta), which functions redundantly in the Krebs cycle, show that the metabolic and structural roles of A-S beta in spermatids can be uncoupled, highlighting a moonlighting function of this Krebs cycle component in CRL activation.

• 出版日期2016-4-4