Aberrant expression of HOXA9, DEK, CBL and CSF1R in acute myeloid leukemia

作者：Casas S; Nagy B; Elonen E; Aventin A; Larramendy ML; Sierra J; Ruutu T; Knuutila S^*

来源：Leukemia and Lymphoma, 2003, 44(11): 1935-1941.

DOI：10.1080/1042819031000119299

• quantitative real-time RT-PCR
• acute myeloid leukemia
• gene expression
• array-based methods

Previous gene function analyses have indicated that HOXA9 , DEK , CBL and CSF1R are aberrantly expressed in acute myeloid leukemia (AML). We analyzed the expression of these genes in a series of 41 adult patients with AML using quantitative real-time RT-PCR, and tested the association of the expression with the following hematologic and clinical parameters: age, FAB, immunophenotype and karyotype aberrations. A high proportion of the patients showed over- or underexpression of the analyzed genes. DEK was overexpressed in 98% of the cases, whereas CBL , CSF1R and HOXA9 were either overexpressed in 20%, 17% and 78% or underexpressed in 20%, 42% and 15% of the cases, respectively. Patients whose karyotype contained t(8;21)(q22;q22), showed lower relative expression of HOXA9 at a statistically significant level ( p <0.05). Bone marrow samples without expression of CD34 antigen were associated with either overexpression of DEK or HOXA9 . Furthermore, an association was found between the AML-M2 subtype and lower expression of CBL , CSF1R or HOXA9 , and between the AML-M5 subtype and CBL or CSF1R overexpression.

• 出版日期2003-9