科研之友
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摘要
Single nucleotide polymorphisms are implicated as having a significant role In regulating growth, development and, thereby, human health and disease. We have developed a method for Identifying single nucleotide genetic alterations by combining hairpin-forming DNA probes and electrochemical detection of sandwich DNA hybridization. Incorporation of hairpin-forming competitor probes and the catalyzed reporter deposition amplification system further Improves assay specificity by 7-fold and sensitivity by 100-fold. We have demonstrated that the system successfully identified the factor V Leiden mutations from human blood specimens.
- 出版日期2002-6-15
