A simple surface plasmon resonance biosensor for detection of PML/RAR alpha based on heterogeneous fusion gene-triggered nonlinear hybridization chain reaction

作者：Guo, Bin; Cheng, Wei; Xu, Yongjie; Zhou, Xiaoyan; Li, Xinmin; Ding, Xiaojuan; Ding, Shijia^*

来源：Scientific Reports, 2017, 7(1): 14037.

DOI：10.1038/s41598-017-14361-5

• ACUTE PROMYELOCYTIC LEUKEMIA
• ULTRASENSITIVE DETECTION
• IMMOBILIZATION-FREE
• DNA HYBRIDIZATION
• RAPID DIAGNOSIS
• NUCLEIC-ACIDS
• RARA
• TRANSLOCATION
• AMPLIFICATION
• NANOSTRUCTURES

In this work, a simple and enzyme-free surface plasmon resonance (SPR) biosensing strategy has been developed for highly sensitive detection of two major PML/RAR alpha (promyelocytic leukemia, retinoic acid receptor alpha) subtypes based on the heterogeneous fusion gene-triggered nonlinear hybridization chain reaction (HCR). On the gold chip surface, the cascade self-assembly process is triggered after the introduction of PML/RAR alpha. The different fragments of PML/RAR alpha can specifically hybridize with capture probes (Cp) immobilized on the chip and the hybridization DNA1 (H-1). Then, the nonlinear HCR is initiated by the complex of Cp-PML/RAR alpha-H-1 with the introduction of two hybridization DNA chains (H-2 and H-3). As a result, a dendritic nanostructure is achieved on the surface of chip, leading to a significant SPR amplification signal owing to its high molecular weight. The developed method shows good specificity and high sensitivity with detection limit of 0.72 pM for "L" subtype and 0.65 pM for "S" subtype. Moreover, this method has been successfully applied for efficient identification of clinical positive and negative PCR samples of the PML/RAR alpha subtype. Thus, this developed biosensing strategy presents a potential platform for analysis of fusion gene and early diagnosis of clinical disease.

• 出版日期2017-10-25
• 单位重庆医科大学