Advanced oxidation protein products induce apoptosis of human chondrocyte through reactive oxygen species-mediated mitochondrial dysfunction and endoplasmic reticulum stress pathways

作者：Ye, Wenbin; Zhu, Siyuan; Liao, Congrui; Xiao, Jun; Wu, Qian; Lin, Zhen; Chen, Jianting^*

来源：Fundamental & Clinical Pharmacology, 2017, 31(1): 64-74.

DOI：10.1111/fcp.12229

• advanced oxidation protein products
• apoptosis
• endoplasmic reticulum stress
• human chondrocyte
• mitochondria membrane potential
• reactive oxygen species

Advanced oxidation production products (AOPPs) have been confirmed to accumulate in patients with rheumatoid arthritis (RA). Previous study demonstrated that AOPPs could accelerate cartilage destruction in rabbit arthritis model. However, the effect of AOPP stimulation on apoptosis of human chondrocyte and the underlying mechanisms remains unclear. This study demonstrated that exposure of chondrocyte to AOPPs resulted in cell apoptosis. AOPP stimulation triggered reactive oxygen species (ROS) production, which induced mitochondrial dysfunction and endoplasmic reticulum stress (ER stress) resulted in caspase activation. Furthermore, an antioxidant, N-acetylcysteine, markedly blocked these signals. Our study demonstrated that AOPPs induce apoptosis via ROS-related mitochondria- and ER-dependent signals in human chondrocyte. Targeting AOPP-triggered ROS generation might be as a promising option for patients with RA.

• 出版日期2017-2
• 单位南方医科大学