The Human Mitochondrial tRNA(Met): Structure/Function Relationship of a Unique Modification in the Decoding of Unconventional Codons

作者：Bilbille Yann; Gustilo Estella M; Harris Kimberly A; Jones Christie N; Lusic Hrvoje; Kaiser Robert J; Delano Michael O; Spremulli Linda L; Deiters Alexander; Agris Paul F^*

来源：Journal of Molecular Biology, 2011, 406(2): 257-274.

DOI：10.1016/j.jmb.2010.11.042

• 5-formylcytidine
• NMR structure
• mitochondrial translation
• codon expansion
• near cognate decoding

Human mitochondrial mRNAs utilize the universal AUG and the unconventional isoleucine AUA codons for methionine. In contrast to translation in the cytoplasm, human mitochondria use one tRNA, hmtRNA(Met)CAU, to read AUG and AUA codons at both the peptidyl- (P-), and aminoacyl- (A-) sites of the ribosome. The hmtRNA(Met)CAU has a unique post-transcriptional modification, 5-formylcytidine, at the wobble position 34 (f(5)C(34)), and a cytidine substituting for the invariant uridine at position 33 of the canonical U-turn in tRNAs. The structure of the tRNA anticodon stem and loop domain (hmtASL(Met)CAU), determined by NMR restrained molecular modeling, revealed how the f(5)C(34) modification facilitates the decoding of AUA at the P- and the A-sites. The f(5)C(34) defined a reduced conformational space for the nucleoside, in what appears to have restricted the conformational dynamics of the anticodon bases of the modified hmtASL(Met)CAU. The hmtASL(Met)CAU exhibited a C-turn conformation that has some characteristics of the U-turn motif. Codon binding studies with both Escherichia coli and bovine mitochondrial ribosomes revealed that the f(5)C(34) facilitates AUA binding in the A-site and suggested that the modification favorably alters the ASL binding kinetics. Mitochondrial translation by many organisms, including humans, sometimes initiates with the universal isoleucine codons AUU and AUC. The f(5)C(34) enabled P-site codon binding to these normally isoleucine codons. Thus, the physicochemical properties of this one modification, f(5)C(34), expand codon recognition from the traditional AUG to the non-traditional, synonymous codons AUU and AUC as well as AUA, in the reassignment of universal codons in the mitochondria.

• 出版日期2011-2-18