Antioxidant activity and cytotoxicity against cholangiocarcinoma of carbazoles and coumarins from Clausena harmandiana

作者：Songsiang Uraiwan; Thongthoom Tula; Zeekpudsa Pornsilp; Kukongviriyapan Veerapol; Boonyarat Chantana; Wangboonskul Jinda; Yenjai Chavi^*

来源：ScienceAsia, 2012, 38(1): 75-81.

DOI：10.2306/scienceasia1513-1874.2012.38.075

• lipid peroxidation
• antioxidants
• free radicals
• natural products
• drug development

Nine carbazoles and three coumarins were isolated from C. harmandiana. Crude extracts and all isolates were evaluated for antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and lipid peroxidation assay and cytotoxicity against cholangiocarcinoma, KKU-OCA17 and KKU-214 cell lines. 7-Hydroxyheptaphylline and nordentatin showed strong antioxidant activity on lipid peroxidation with IC50 values of 2.95 and 2.90 mu M respectively, while the activity on a DPPH assay showed IC50 values of 56.82 and 29.3 mu M, respectively. The two compounds exhibited strong cytotoxicity against KKU-OCA17 (IC50=88.7 and 46.1 mu M, respectively) and KKU-214 (IC50=43.7 and 39.1 mu M, respectively) cell lines. The results show convincingly that these compounds may be the promising leads for the development of cytotoxic agents.

• 出版日期2012-3