In vivo cloning of a carboxy-terminal rpoB allele which confers altered transcriptional properties

作者：Rowland GC; Lim PP; Glass RE

来源：Folia Microbiologica, 1995, 40(6): 588-594.

DOI：10.1007/BF02818514

A 3'-terminal mutation of the gene encoding the a subunit of Escherichia coli RNA polymerase was isolated using an in vivo polA(Ts) technique. Cloning of the allele was monitored by virtue of the fact that the deletion Delta(rpoB)1570-1 resulted in an altered-size restriction fragment. DNA sequencing confirmed the predicted nature and location of the mutation: Delta(rpoB)1570-1 involved an in-frame deletion of 186 bp (62 codons) encoding amino acid residues 967-1028. The phenotype conferred by Delta(rpoB)1570-1 is discussed with respect to conserved domains within the beta polypeptide.

• 出版日期1995