Development of a novel noninvasive adhesive patch test for the evaluation of pigmented lesions of the skin

作者：Gerami Pedram^*; Alsobrook John P II; Palmer Tara J; Robin Howard S

来源：Journal of the American Academy of Dermatology, 2014, 71(2): 237-244.

DOI：10.1016/j.jaad.2014.04.042

• dysplastic nevi
• melanocytic neoplasms
• melanoma
• molecular signatures
• nevi

Background: The accurate clinical assessment of melanocytic neoplasms is a challenge for clinicians. Currently, obtaining a biopsy specimen and conducting a histologic examination is the standard of care. The incidence of melanoma in white populations is high, resulting in a large number of biopsy specimens.
Objective: The objective of this study is to develop a noninvasive genomic method using mRNA to classify pigmented skin lesions as either benign or malignant.
Methods: An adhesive patch method was used to obtain cells from the surface of melanocytic lesions. mRNA was extracted and a genomic signature was formulated in a training set of benign and malignant melanocytic neoplasms and subsequently tested in a validation set.
Results: A 2-gene signature assessing the expression levels of CMIP and LINC00518 was able to differentiate melanomas from nevi in an independent validation set of 42 melanomas and 22 nevi with a sensitivity of 97.6% and specificity of 72.7%.
Limitations: Larger and more diverse sets of melanomas and nevi are needed for additional validation of the molecular expression profiling in various subsets of melanocytic neoplasms.
Conclusion: Our data suggest that mRNA molecular signatures can serve as a highly useful noninvasive method of differentiating melanoma from nevi and decrease the number of unnecessary biopsies.

• 出版日期2014-8
• 单位西北大学