Alterations in the MA and NC Domains Modulate Phosphoinositide-Dependent Plasma Membrane Localization of the Rous Sarcoma Virus Gag Protein

作者：Nadaraia Hoke Shorena; Bann Darrin V; Lochmann Timothy L; Gudleski O'Regan Nicole; Parent Leslie J^*

来源：Journal of Virology, 2013, 87(6): 3609-3615.

DOI：10.1128/JVI.03059-12

Retroviral Gag proteins direct virus particle assembly from the plasma membrane (PM). Phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P-2] plays a role in PM targeting of several retroviral Gag proteins. Here we report that depletion of intracellular PI(4,5)P-2 and phosphatidylinositol-(3,4,5)-triphosphate [PI(3,4,5)P-3] levels impaired Rous sarcoma virus (RSV) Gag PM localization. Gag mutants deficient in nuclear trafficking were less sensitive to reduction of intracellular PI(4,5)P-2 and PI(3,4,5)P-3, suggesting a possible connection between Gag nuclear trafficking and phosphoinositide-dependent PM targeting.

• 出版日期2013-3